Comparison Of The “Death By Landscape” And “The Yellow

Comparison of the â€Å"Death by Landscape† and â€Å"The Yellow Wallpaper† Terror, a feeling shared by all beings with emotions, With such an influence in the well-being of a human being, terror is something that creates interest and research while repelling and frightening an individual. The gothic literature is an evoked presence of terror by humans to create a sense of confusion with a reader leaving them with no explanation of the unrealistic events and removing the ability of control and comprehension. Margaret Atwood and Charlotte Perkins Gilman master the unexplained supernatural and unrealistic events in their own stories, â€Å"Death by Landscape† and â€Å"The Yellow Wallpaper†. The first follows the abrupt and unexplained loss of the main†¦show more content†¦This is due to the state that Lois is in throughout the story. Lucy is not alive in a body, but in the obsession of Lois’s mind. Society deals with death in many ways, whether it is in ritual or locking it behind a wall in the mind. Lois locks Lucy in the depths of her perception and mind as shown by the paintings she has collected, â€Å"every one of them is a picture of Lucy. You can’t see her exactly. But she’s there, in behind the pink stone island or the one behind that.† (Atwood 129). Due to the disturbance that Lois is dealing with it’s explainable that this is Lois’s way of coping with Lucy’s death. However this end doesn’t solve the question the reader frequently asks. Where, why and how did Lucy die? The questions which cannot be answered neither by the reader or Lois. Leaving out the answer creates the dreadful terror that humans pursue, the answer of death and it’s unexplainable control of events. The temporary feeling and atmosphere of terror that is throughout the story exemplifies the unexplained supernatural by showing Lois as a victim of an event she has no comprehension or power over. She is affected to a point where â€Å"she would never go up north†¦ to any place with wild lake and wild trees and the calls of loons. (Atwood 129). In the rugged landscape of the wilderness, it is equal to death where in that the wild is very similar to the uncontrollable nature of death. Lucy’s presence is emphasized by her absence as she continuesShow MoreRelatedThe Character Of Conrad And Cormac Conrads Heart Of Darkness1640 Words   |  7 Pagesconditions without explicit statements, authors cannot elucidate each event and character’s thought while continuing to hold the reader’s attention. Therefore, many authors utilize the landscape of the story to parallel a character or group of characters. In Heart of Darkness, author Joseph Conrad personifies the la ndscape as a fluid character. Consequently, as the reader begins to further comprehend the landscape’s meaning, Marlow’s understanding of the people around him becomes more clear. LikewiseRead MoreSeated Bodhisattva Avalokitesvara ( Guanyin )982 Words   |  4 Pagesstatue was made during the Tang dynasty. While, the later statue was made during the Northern Song dynasty. These statues were chosen because the same deity is depicted and were made in a similar geographic and cultural location. This allows for the comparison of the different styles between the 2 periods. The statue made during the Northern Song dynasty depicts the Bodhisattva Avalokitesvara sitting in a pose called the relaxed pose or easy pose. This statue also depicts the Bodhisattva adorned in lavishRead MoreCircus Posters of P.T. Barnum and The Ringling Brothers1154 Words   |  5 Pages1881 Barnum, James Bailey, and James Hutchinson partnered up to create P.T. Barnum’s Greatest Show on Earth The Great London Circus, which later became Barnum Bailey’s Greatest Show on Earth in 1888 (P.T. Barnum). 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TheRead Moreâ€Å"Starry Night† Is One Of The Most Popular Canvasesin Modern-Day1385 Words   |  6 Pagessilence of the village which is supposed to be Van Gogh’s hometown Netherland. The painting also consists of a cypress tree which is typically found in graveyards . This might have something to do with the artist’s perception of the inevitability of death and how close it is on Earth. Starry Night is just one example of Vincent Van Gogh’s numerous painting which show night life, from which it can be presumed that the artist loved night time. Vincent van Gogh s art is generally observed by criticizersRead MoreRhetorical Analysis Essay848 Words   |  4 Pagesused a metaphor comparing his grandmother to the Rainy Mountain. For example, he writes that â€Å"[a]lthough my grandmother lived out her long life in the shadow of Rainy Mountain, the immense landscape of the continental interior lay like memory in her blood (Momaday 131). This metaphor compares the immense landscape of the Rainy Mountain’s continental interior to his grandmother’s memory instilled in her bloodstream. By using metaphors, Momaday reminds young individuals of their traditional life by comparingRead More Comparing Symbols and Symbolism in Blue Hotel, Black Cat, Night, Alfred Prufrock, Red Wheelbarrow1620 Words   |  7 Pagesspecifically dream psychology, red may symbolize sexuality, passion, anger, revolution or danger. Red being the color of blood, it is the symbol of life, which consequently is why Hindu and Chinese brides wear red. But, blood may also represent injury or death. (Ackroyd 253)    Blue is the color of intellect, peace and contemplation. It represents water and serenity, and symbolizes the sky and infinity. 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Both Joseph Conrad, author of Heart of Darkness, and Cormac McCarthy, author of The Road, manipulate the landscape to represent the human condition. In Heart of Darkness, Conrad personifies the landscape as a fluid character, so as the reader begins to further comprehend the landscape’s meaning, Marlow’s understandingRead MoreComparing The Girl With A Pearl Earring And Mona Lisa1332 Words   |  6 PagesName Professor Course Date Comparison of the Girl with a Pearl Earring and Mona Lisa   The Girl with a Pearl Earring and Mona Lisa are the two most famous portraits ever painted  (Courtauld 36). The girl with a pearl earring follows the Mona Lisa painting, which is considered as the most famous painting. The girl with a pearl earring, by Johannes Vermeer, and the Mona Lisa by Leonardo da Vinci were created during the same historical time. Historians believe that Leonardo da Vinci created his art

Lord Atkin s Neighbor Principle - 1442 Words

As illustrated through Lord Atkin’s â€Å"Neighbour Principle†, established in the case of Donoghue v Stevenson , tort of negligence provides a remedy for those who suffer loss due to the behaviour of a person who fails to adhere to a legal duty to take reasonable care. On these grounds, it appears that Geoff and Beth may be held to have joint several liability in negligently causing Paul physical damage. Paul could bring a tortuous action against Geoff on the grounds of negligent driving, in relation to his head and collarbone injuries. As elucidated by Greer LJ in Haynes v Harwood , to have a cause of action in negligence, there must be the neglect of â€Å"some duty owed to the† claimant. Accordingly, Geoff’s legal duty of care is established through s.3 Road Traffic Act 1988, which, in imposing strict liability in cases of negligent driving, creates a duty of care. It provides that a driver is deemed to have committed a tortuous act by driving carelessly â€Å"without due care and attention† , therefore as Geoff is driving a mechanically propelled vehicle, he has a statutory duty of care towards other road users. There then arises the question as to whether Geoff’s behaviour has fallen below the â€Å"reasonable standard of care†. This is based on the premise that liability arises where the defendant causes loss or damage to another â€Å"by fault†. Paul could thus attest the first two hurdles in bringing a negligence action against Geoff, as Geoff has clearly breached his statutory duty ofShow MoreRelatedCase Study of Negligence1406 Words   |  6 Pagesthe Bright Smiles Dental Surgery) owned duty of care to plaintiff (Tony)? 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The Stupidest Angel Chapter 21 Free Essays

Chapter 21 AVENGING ANGEL Oh shit, oh shit, oh shit, oh shit, Theo thought. He twisted his ankle when he hit the ground; pain shot up his leg like liquid fire. He fell and rolled onto his back in the mud. We will write a custom essay sample on The Stupidest Angel Chapter 21 or any similar topic only for you Order Now He’d pushed the remote button that unlocked the Range Rover too soon the vehicle had chirped and the lights had blinked, alerting the undead. He’d made the jump blind, and missed. They were coming for him. He pushed himself up and started hopping toward the Range Rover the car key ready in his right hand, his flashlight lost behind him in the mud. Grab him, you rotting fucks,† screamed Dale Pearson. Theo fell forward as his good foot slipped out from under him, but he rolled back to his feet, a bolt of pain shooting white-hot across his shin. He caught himself on the back window of the black Range Rover, snatching at the rear wiper for balance. He risked a glance back toward his pursuers and heard a loud thump by his head followed by a deafening screech. He turned just in time to see a skeletal woman sliding across the roof of the Range Rover, leading with her teeth. He ducked, but not before he felt fingernails raking his neck, teeth thumping into his scalp. She rode him to the ground and he could feel a grating pain in his head as the zombie tried to bite through his skull. His face was pushed into the mud. His nostrils and mouth filled with water, and amid a flashing whiteness of terror he thought, I’m so sorry, Molly. â€Å"Yuck! That’s hideous!† said Bess Leander, spitting a couple of teeth on the back of Theo’s head. Marty in the Morning grabbed Theo by the head and licked the teeth marks that Bess had left. â€Å"That’s horrible. He’s stoned. I’m not eating stoned brains.† The undead moaned in disappointment. â€Å"Get him up,† said Dale. Theo inhaled a great spray of mud along with his first breath and he went into a coughing fit as the undead lifted him up and held him against the back window of the Range Rover. Someone wiped the mud out of his eyes, and a stench that made him gag filled his nostrils. He could see the dead but animated face of Dale Pearson only inches from his own. The corpse’s foul breath overwhelmed him. Theo tried to twist away from the evil Santa, but decaying hands held his head fast. â€Å"Hey, hippie,† said Dale. He held Theo’s flashlight down by his Santa beard to illuminate his face from below. There were two stripes of bloody drool running down either side of the beard. â€Å"You’re not thinking that your pot-smoking ways are going to save you, are you? Don’t.† He pulled a snub-nose revolver out of the pocket of his red coat and shoved it under Theo’s chin. â€Å"We’ll have plenty to eat. We can afford to waste you.† Dale ripped open the Velcro fasteners of Theo’s jacket and started feeling around his waist. â€Å"No gun? You suck as a lawman, hippie.† He went to the pockets of Theo’s cop shirt. â€Å"But this! The one thing you can be depended on for.† Dale held up Theo’s lighter, then reached in, tore the whole pocket off the cop shirt, and wrapped the dry fabric around the lighter. â€Å"Marty, try this. Keep it dry.† Dale gave the lighter to a rotting guy with a wet Ziggy Stardust red mullet, who slogged back to the pile of debris at the side of the chapel. Theo watched as Marty in the Morning bent over the pile of plywood, pine branches, two-by-fours, cardboard, and the torn-up body of Ben Miller. The wind was still whipping, the rain less intense now, but even so the drops stung Theo’s face when they hit. Don’t light, don’t light, don’t light, Theo chanted in his head, but then his hope drained away when he saw the orange flame catch on the debris, and Marty in the Morning pull away with his sleeve on fire. Dale Pearson stepped aside so Theo could see the fire whipping up the side of the building, then put the .38 against Theo’s temple. â€Å"Take a good look at our little barbecue fire, hippie. It’s the last thing you’re going to see. We’re gonna eat your crazy wife’s brains char-broiled.† Theo smiled, happy that Molly wasn’t inside, wouldn’t be part of the massacre. â€Å"I didn’t hear ‘Shave and a Haircut, † said Ignacio Nunez. â€Å"Did you hear ‘Shave and a Haircut’?† Tuck panned his flashlight across a dozen frightened faces, then one whole side of the chapel went orange with the light from the fire outside the windows. One woman screamed, others stared in horror as smoke started to snake in around the window frames. â€Å"Change of plan,† Tuck said. â€Å"We go now. Guys in front of your groups. Give the car keys to the next person back.† â€Å"They’ll be waiting for us,† said Val Riordan. â€Å"Fine, you burn up,† Tuck said. â€Å"Guys, knock over whatever gets in your way, everyone behind just keep going for the cars.† All the barricades and braces had been removed from the chapel doors. Tuck put his shoulder against one door, Gabe Fenton was on the other. â€Å"Ready. One, two, three!† They threw their shoulders against the doors and bounced back into the others. The doors had only opened a couple of inches. Someone shone a flashlight through the gap to reveal a huge pine-tree trunk braced against one of the doors. â€Å"New plan,† shouted Tuck. Theo tried to look at the fire, but he couldn’t see beyond the undead eyes of Dale Pearson. Thought had fled. There was just fear and anger and the pressure of the gun barrel against his temple. He heard a whooshing sound and a thump by his ear and the gun barrel was gone. Dale Pearson was stepping away from him, holding a dark stump where his gun hand had just been. Dale opened his mouth to shout something, but in that second a thin line appeared across his face at nostril level and half of his head slid to the ground. He slumped in a pile at Theo’s feet. The hands that were holding Theo let go. â€Å"Brains!† screamed one of the undead. â€Å"Crazy-woman brains!† Theo fell on top of Dale’s rekilled body, then spun around to see what was happening. â€Å"Hi, honey,† Molly said. She stood on the roof of the Range Rover, grinning, wearing a leather jacket, sweatpants, and her red Converse All Stars, holding the ancient Japanese sword in Hasso No Kamae before her, the blade gleaming orange in the light from the burning church. There was a dark swath across the blade where it had hewn the head of the zombie Santa. Theo had never been a religious man, but he thought in that instant that this must be what it was like to look on the face of an avenging angel. The zombies who had been holding Theo reached for Molly’s legs, and in a single motion she stepped back and brought the sword around in a low arc that sent a rain of severed hands flying into the mud. The undead wailed around her, and tried to claw their way onto the SUV with their stumps. Bess Leander tried to replicate the move she had used on Theo, stepping up the hood behind Molly and diving across the roof of the Range Rover. Molly spun and sidestepped, making a low swing with her sword that would have not looked out of place on a golf course. Bess’s head rolled off the top of the Land Rover into Theo’s lap. He pushed it aside and got to his feet. â€Å"Honey, you might want to go let everyone out of the chapel before they burn up,† said Molly. â€Å"I’m not sure you want to watch this.† † ‘Kay,† said Theo. The undead had left their stations at the front and back doors of the chapel, where they had been waiting to ambush the escaping partyers, and charged Molly. Three fell headless while Molly stood on the Land Rover, but as they surrounded her, she ran and leaped over the heads of the mob, landing behind them. Theo ran for the front doors of the chapel, his vision blurred from the rain and the blood running into his eyes from the bite wound on his head. He looked back for a second and saw Molly sailing over the top of her attackers. He nearly ran into two great pine logs that had been braced against the chapel doors. He looked back a second and caught a glimpse of Molly mowing down two more zombies, one split down the middle from the crown of his head to his sternum, then he turned and tried to get his back under one of the logs. â€Å"Theo, is that you?† Gabe Fenton had his face pressed in an inch-wide gap between the doors. â€Å"Yeah. There are logs against the doors,† Theo said. â€Å"I’m going to try to move them.† Theo took three deep breaths and lifted for all he was worth, feeling as if veins would explode in his temples. The wound on his head throbbed with every heartbeat. But the tree trunk moved a couple of inches. He could do this. â€Å"Is it working?† yelled Gabe. â€Å"Yeah, yeah,† said Theo. â€Å"Give me a second.† â€Å"It’s filling up with smoke in here, Theo.† â€Å"Right.† Theo strained again and the log moved another two inches to the right. Another foot and they’d be able to get the door open. â€Å"Hurry, Theo,† said Jenny Masterson. â€Å"It’s – † She went into a coughing fit and couldn’t finish what she was saying. Theo could hear everyone coughing inside. Wails of rage and pain were coming from the side of the chapel where Molly was fighting. She must be okay, they were still yelling about eating her brain. Another lift, another two inches. Gray smoke was streaming out the crack between the doors. Theo fell to his knees with the effort and almost passed out. He shook himself back into consciousness, and as he prepared to put his back into another effort, hoping it wouldn’t be his last, he noticed that the screaming had stopped at the side of the chapel. Rain, wind, the coughing of the trapped, and the crackle of the fire. That was all he heard. â€Å"Oh my God. Molly!† he screamed. A hand on his cheek, a voice at his ear. â€Å"Hey, sailor, need a little hand getting your church door open, if you know what I mean?† Sirens sounded in the distance. Someone had seen the burning chapel through the storm and had somehow gotten through to the volunteer fire department. The Lonesome Christmas survivors were gathered in the middle of the parking lot, illuminated by headlights. The heat from the fire had driven them nearly seventy-five yards to the street. Even this far away, Theo could feel the heat on his cheek from the fire as Lena Marquez bandaged his head. Others sat in the open hatchbacks of SUVs, trying to catch their breath after being exposed to the smoke, drinking bottled water or just lying there dazed. Around the burning chapel the wet pine forest steamed, a great white cloud rising into the sky. Down the left side of the chapel: carnage – a rekilling ground of the undead, where Molly had hacked them into submission, even chasing down the last few in the woods and decapitating them after she and Theo had let the partyers out of the chapel. Molly sat beside Theo, under the open hatchback of someone’s Expedition. â€Å"How did you know?† he asked. â€Å"How could you possibly have known?† â€Å"The bat told me,† Molly said. â€Å"You mean he showed up and you said, ‘What’s wrong, boy, is Timmy trapped down a well? and he barked to tell you that’s what was wrong? Like that?† â€Å"No,† Molly said. â€Å"It was like, ‘Your husband and a bunch of other people have barricaded themselves in the chapel against a horde of brain-eating zombies and you need to go save them. Like that. He has some kind of accent. Sounds Spanish.† â€Å"I for one am glad that you went off your meds,† said Tucker Case, who was standing next to Lena as she bandaged Theo’s head. â€Å"A few hallucinations are a small price to pay, if you ask me.† Molly held up her hand for him to be quiet. She stood and brushed the pilot aside, looking back toward the burning church. A tall dark figure in a long coat was coming toward them through the killing field. â€Å"Oh no,† said Theo. â€Å"Everyone get in the cars and lock them.† â€Å"No,† Molly said, dismissing Theo’s instructions with a distracted backward wave. â€Å"We’re okay.† She met the angel in the middle of the parking lot. â€Å"Merry Christmas,† said the angel. â€Å"Yeah, you, too,† said Molly. â€Å"Have you seen the child? Joshua?† asked Raziel. â€Å"There’s a kid over there with the others,† said Molly. â€Å"That’s probably him.† â€Å"Take me to him.† â€Å"That’s him,† Theo said. â€Å"That’s the robot guy.† â€Å"Shhhhhh,† Molly shushed. Raziel walked to where Emily Barker was holding her son, Joshua, sitting on the back of Molly’s Honda. â€Å"Mom,† wailed Joshua. He hid his face in his mother’s chest. But Emily was still stunned by witnessing her mate’s death, and she didn’t react at all except to hold the boy tighter. Raziel put his hand on the boy’s head. â€Å"Fear not,† he said. â€Å"For I bring you tidings of great joy. Behold, your Christmas wish has been granted.† The angel waved toward the fire and the carnage and the exhausted and terrified survivors as if he were a game-show hostess presenting a washer/dryer set. â€Å"Not what I would have wished for,† the angel said, â€Å"but I am but a lowly messenger.† Josh rolled in his mother’s arms and faced the angel. â€Å"I didn’t ask for this. This isn’t what I wished for.† â€Å"Sure it is,† said Raziel. â€Å"You wished that the Santa you saw killed be brought back to life.† â€Å"No, I didn’t.† â€Å"That’s what you said. You said you wanted him brought back to life.† â€Å"That’s not what I meant,† said Joshua. â€Å"I’m a kid. I don’t always get stuff right.† â€Å"I’ll vouch for that,† said Tucker Case, stepping up behind the angel. â€Å"He is a kid, and he is wrong most of the time.† â€Å"We still should cut your head off,† said Josh. â€Å"See,† said Tuck. â€Å"Always wrong.† â€Å"Well, if you didn’t mean you wanted him brought back to life, what did you mean?† asked Raziel. â€Å"I didn’t mean I wanted Santa to be a zombie and kill big, dumb Brian and everything. I wanted everything to be okay. Like it never happened. So it would be a good Christmas.† â€Å"That’s not what you said,† said Raziel. â€Å"That’s what I wanted,† said Joshua. â€Å"Oh,† said the angel. â€Å"Sorry.† â€Å"So he’s an angel?† Theo said to Molly. â€Å"Like a real angel?† Molly nodded, smiling. â€Å"Not a killer robot?† Molly shook her head. â€Å"He’s here to grant a Christmas wish, to one child.† â€Å"Like it never happened?† the angel asked Joshua. â€Å"Yeah!† said Josh. â€Å"Oops,† said the angel. Molly stepped over and put her hand on the angel’s shoulder. â€Å"Raziel, you fucked up. Fix it?† The angel looked at her and grinned. Perfect teeth, if a little sharp. â€Å"So be it,† he said. â€Å"Glory to God in the highest, peace on Earth, goodwill toward men.† How to cite The Stupidest Angel Chapter 21, Essay examples

Oediups the king Essay Example For Students

Oediups the king Essay To Laius, King of Thebes, an oracle foretold that the child born to him by his queen Jocasta would slay his faher and wed his mother. So when in time a son was born the infants feet were riveted together and he was left to die on Mount Cithaeron. But a shepherd found the babe and tended him, and delivered him to another shepherd who took him to his master, the King or Corinth. Polybus bein childless adopted the boy, who grew up believing that he was indeed the Kings son. Aferwards doubting his parentage he inqured of the Delhic god and heard himself the weird declared before to Laius. Wherefore he fled from what he deemed his fathers house and in his flight he encountered and unwillingly slew his father Laius. Arriving at Thebes he answered the riddle of the Sphinx and the grateful Thebans amde their deliver king. So he reigned in the room of Laius, and espused the widowed queen. Children wer born to them and Thebes prospered under his rule, but again a grievous plague fell upon the city. Again the oracle was consulted and it bade them purge themselves of blood-guiltiness. Oedipus denounces the crime of which he is unaware, and undertakes to track out the criminal. Step by step it is brought home to him that he is the man. The closing scene reveals Jacasta slain by her owm hand and Oedipus blinded by his own act and praying for death or exile. Bibliography:

Wuthering Heights monologue Essay Example For Students

Wuthering Heights monologue Essay A monologue from the novel by Emily Bronte NOTE: This monologue is reprinted from Wuthering Heights. Emily Bronte. New York: Harper Brothers, 1848. MRS. LINTON: How long is it since I shut myself in here? It seems a weary number of hours it must be more. I remember being in the parlour after they had quarrelled, and Edgar being cruelly provoking, and me running into this room desperate. As soon as ever I had barred the door, utter blackness overwhelmed me, and I fell on the floor. I couldn\t explain to Edgar how certain I felt of having a fit, or going raging mad, if he persisted in teasing me! I had no command of tongue, or brain, and he did not guess my agony, perhaps: it barely left me sense to try to escape from him and his voice. Before I recovered sufficiently to see and hear, it began to be dawn, and, Nelly, I\ll tell you what I thought, and what has kept recurring and recurring till I feared for my reason. I thought as I lay there, with my head against that table leg, and my eyes dimly discerning the grey square of the window, that I was enclosed in the oak-panelled bed at home; and my heart ached with some great grief which, just waking, I could not recollect. I pondered, and worried myself to discover what it could be, and, most strangely, the whole last seven years of my life grew a blank! I did not recall that they had been at all. I was a child; my father was just buried, and my misery arose from the separation that Hindley had ordered between me and Heathcliff. I was laid alone, for the first time; and, rousing from a dismal doze after a night of weeping, I lifted my hand to push the panels aside: it struck the table-top! I swept it along the carpet, and then memory burst in: my late anguish was swallowed in a paroxysm of despair. I cannot say why I felt so wildly wretched: it must have been temporary derangement; for there is scarcely cause. But, supposing at twelve years old I had been wrenched from the Heights, and every early association, and my all in all, as Heathcliff was at that time, and been converted at a stroke into Mrs. Linton, the lady of Thrushcross Grange, and the wife of a s tranger: an exile, and outcast, thenceforth, from what had been my world. You may fancy a glimpse of the abyss where I grovelled! Shake your head as you will, Nelly, you have helped to unsettle me! You should have spoken to Edgar, indeed you should, and compelled him to leave me quiet! Oh, I\m burning! I wish I were out of doors! I wish I were a girl again, half savage and hardy, and free; and laughing at injuries, not maddening under them! Why am I so changed? why does my blood rush into a hell of tumult at a few words? I\m sure I should be myself were I once among the heather on those hills. Open the window again wide: fasten it open! Quick, why don\t you move? We will write a custom essay on Wuthering Heights monologue specifically for you for only $16.38 $13.9/page Order now

Oral Communication Essays - Communication, Human Communication

Oral Communication Essays - Communication, Human Communication Oral Communication Once viewed as two separate disciplines, business and communication, have now meshed together to produce a hybrid business environment in which the everyday functions of business are intimately tied to communication (Pincus, 1997). Communication in the business world is imperative for success. This holds true for interpersonal communication, communication between management and staff, and for practically every other contact a business has, both within its own establishment and the outside world. Effective communication is critical for the success of any organization. Through the use of proper communication skills, individuals will be better able to function as a group, thus allowing organizations to share information, analyze situations and to set goals (Nelton, 1995). Communicating properly among peers improves an individuals all around skills. The more successfully a business functions the better it enables employees to perform jobs better. Managers pass on information and train subordinates more effectively, and in general a business has a better chance of profiting. In todays turbulent economic environment and rapid technological change, communication is critical in allowing a business to deal with the restructuring of national and international economies, in preventing market saturation, and in allowing a business to deal with their competitors more effectively (Nelton, 1995, PG). Cushman and King (1997) have proposed the high speed management to describe this new busin ess environment. They emphasize the importance of communication in this theory and conclude that: In the final analysis it is the innovative, adaptable, flexible, efficient, and rapid use of information and communication which allows an organization to reorient rapidly and successfully in a volatile business environment. Another very important factor in the changing business environment is that of globalization (Nelton, 1995). It is very evident when we look at the current state of world affairs that our world is becoming a smaller place. We now have overnight delivery of packages, email communication and the ever so popular cellular communication. Globalization and increased international business can be directly attributed to mass media and mass transit. With new technologies such as videophone, Internet chat and Internet meeting rooms the thought of globalization becomes a reality for even the smallest of companies. The concept of globalization sometimes approaches this change as being one which either should or will result in a complete homogenization of culture and the formation of a unified global community. At the very least globalization will result in a number of distinct border cultures, which are hybrids of interacting cultures. What this means is that the savvy business person not only ha s to be prepared to communicate with those of his or her own culture but also with other cultures (Nelton, 1995). Many obvious precipitators of this increased business contact between the worlds cultures can be attributed to this globalization phenomenon. One of the reasons is international agreements such as the North American Free Trade Agreement. The North American Free Trade Agreement was initiated between the United States, Canada, and Mexico on January 1, 1994. This agreement referred to as the trade agreement has had a huge impact on exchange of material and cultural goods between the United States and other nations in North America as well as on the degree of business communication which occurs between these countries. Increased business diversity is not only occurring because of factors such as the North American Free Trade Agreement, it is also occurring because of a greater number of cultures within business itself. Women in the workplace are also making the work force more diversified and increasing the need for more effective communication skills (Nelton, 1995). It is an acknowledged fact that conversational styles and communication skills vary between cultures and genders (Nelton, 1995). It has been noted regarding the increased business contact between cultures as a result of globalization; increased diversity in the workplace itself, whether through the presence of an increased number of cultures or through the presence of a greater number of women; businesses must now devote greater amounts of effort toward communication in recognition of the different communication styles which exist (Nelton, 1995). Deborah Tannen, author of Talking from Nine to Five states: Each individual has a unique style, influenced by a personal history of many influences such as geographic region, ethnicity, class, sexual

Urbanized film by Gary Hustwit 'summary' Essay Example | Topics and Well Written Essays - 500 words

Urbanized film by Gary Hustwit 'summary' - Essay Example The importance of maintaining a proper balance between the natural beauty and the construction of infrastructure is also highlighted in the movie. Urbanized explains the fact that the movement of people to cities has highly increased over the years. It presents the viewer with the fact that by the year 2050, 75 percent of the people of the world would be residing in cities. This reveals the drastic increase in comparison to the year 2010 where it was seen that 50 percent of the world’s population was in cities. With the presentation of the statistics, the maker of the movie tries to explain the importance of the proper construction and maintenance of cities as most of the world’s population would be residing in them. The movie presents the fact that good and effective constructions programs in the cities can help the people living in the city overcome their miseries and live in a better environment. The movie shows the viewer the different cities across the globe to explain the importance of proper construction and architectural strategies for the maintenance of cities. It takes the viewer to the poor, unhygienic conditions of cities like Mumbai in India to show the poor slum conditions prevailing in the cities and very low standards of sanitation and public health as up to 600 people need to use a one washroom. On the other hand, Huswit takes us to Santiego in Chile where proper planning by Alejandro Aravena who is an architect by profession has led to the designing of a housing scheme for people who are non-affording so that they have a better standard of living. He contrasts and compares different cities to indicate the impact that proper planning and construction can have on the living stand ards of the people. Gary Huswit uses his movie â€Å"Urbanized† to explain to the audiences that proper architectural designing, good schemes as well maintenance of sanitation and infrastructure is central to the proper running of a

Project Planning and Project Managment Research Paper

Project Planning and Project Managment - Research Paper Example Despite the risks, project management ensures that tasks are completed successfully to their full requirements. Project management should be started earlier before committing resources and continue to the completion of the task. Companies face numerous opportunities to make their projects viable. There are also numerous techniques that can be used in making the project a success. However, successful project management requires the application of good principles rather than implementing available techniques. The principles are normally derived from experience and are valid across all projects. The success of any project is dependent on how a company applies the principles of projects management. The principles of project management include: a) Identify your business and mind your own business. Sigma has its business of providing information technology solutions across the world. The company should then make a selection of projects that are viable to the business. According to research studies, half of project management is about paying attention. Sigma should learn and apply the best practices. b) Understand customer needs and requirements and put them under version control. Sigma should gather comprehensive information on customer requirements and document the requirements under version control. ... d) Create a competent team with clear tasks. The projected team should include trustworthy and competent members. The team should have their tasks well defined and provided with the necessary working tools. Sigma is expected to offer training to team members to ensure that the project is carried out according to plans. The team should have a team leader and any misfits within the group should be removed. e) Make a follow up on progress and conduct reviews. Projects may have faults and this can only be identified by tracking the progress of the project and review the progress frequently to identify potential loopholes that may be present in the project. The progress should be viewed in a very wide spectrum and pay attention to indicators in order to identify problems before they go out of hand. f) Use baseline controls. Managing the project requires a cost and schedule baseline tracking in handling changes. The changes should be managed deliberately. Sigma is expected to measure probl em areas and apply quantitative methods in tracking the progress of the project towards providing solutions to the identified problems. g) Note down important information and share it among team members. It is important to record requirements, design and management structure and the plan for the project to ensure the improvement of concepts and ideas. Proper documentation ensures that the project has baseline controls and a process that can be revisited. Decision and their rationale should be recorded for future application. h) Conduct tests on various cases. A project work requires decisions that have been tested and their potential outcome known. This reduces uncertainty in conducting some activities. Sigma is expected to develop test cases early enough in order to

History and the United States Essay Example | Topics and Well Written Essays - 1500 words

History and the United States - Essay Example American took most of the Spanish colonies including Cuba with the aim of establishing a favorable place to invest the capital. United States succeeded for the first time in Manila, Philippines and not in Cuba. U.S. used this as a claim that they were assisting Cuba gain its independence, as well as, acquire territory from Spain. The battle lasted a few months where Spain gave in to the conditions that were set by the United States of America. Filipinos were in dire need of independence during that period; they ended up in war with the United States that continued to the early 1900. The United States of America thought that it was their mandate to and right to rule over the people of Philippines. The reason for the notion by the United States was to pass civilization to Filipinos, but mainly for economic advancement. Their brought about a thought â€Å"white man’s burden†; it was aimed at assisting the citizens of Philippines attain the same living standards and civiliz ation, which the Americans have been benefiting from. It was a forced idea because it did not matter whether they were interested with it or not. During America’s first endeavor to acquire colonies; it lost a lot of money, wasted time and military force. However, United States was determined to maintain Philippines as its colony because during the period it reigned over it, their trade links with Europe strengthened. Moreover, United States was able to trade with Asian countries across the Pacific; it came about because they had full control over Guam and Philippines. America’s economy advanced because it traded with China whose population was greater than that of Europe and United States when combined. It was an achievement for the United States because their products and investments were accessible by larger groups of people. America’s colonies provided the opportunity for trade; United States ended up controlling the entire Asian territory. Imperialism was ex perienced during this time when the United States wanted to advance their economic power, although naturally. United States became an imperial power, which was enhanced by its economic gain, wealth and fortune. The war between America and Spain gave a favorable opportunity to the United States to take control over several lands: Carolina, Marshall and Mariania (Guam) islands and Puerto Rico. This war resulted into a stronger United States empire and the collapse of Spanish empire. America gained a political power as a result of imperialism because it was the only country with authority and power over its colonies. The military power was wasted because most of them were killed; a lot of resources were destroyed, which weakened the economic power of the state. New cultural practices were introduced to United States because while they were trading with Asians, Filipinos and the Spanish, they acquired new ways of doing things. In response to Imperialism, most of the countries they were under U.S. target entered into war because they were determined to rescue their country from colonial rule. Cuba, Spain and Asian fought with United States. World War II and Cold war World War II was experienced by many countries globally that took place between 1939 and 1945. Germany is being blamed for the establishment of the war, which resulted from its invasion into Poland. Other

Business health check in hospitality industry

Business health check in hospitality industry 1.0 Introduction 1.1 Aim of the assignment   Ã‚  Ã‚  Ã‚  Ã‚  This report is all about business health check in hospitality industry and finding the gaps of the organisation. After finding the gaps giving the appropriate recommendation and giving the action plan as well as time spam. After that how much it will cost to your organisation to implement that action plan.   Ã‚  Ã‚  Ã‚  Ã‚  As presently I am not working any hospitality related industry therefore I chose to do business health check in Kohinoor continental where I completed my training. 1.2 Organisational introduction The Kohinoor Continental is located at Andheri (East), at a stones throw from Mumbais International Airport and in the midst of the newly developing Business District. It is indeed rare that there exists a business hotel, which enjoys a distinguished patronage such as ours. Therefore during our period of renovation, much as we would have liked to, we could not offer you our hospitality. But now youll agree that the new Kohinoor Continental was well worth the wait. From the moment you enter, youll see that we have spared no expense in order to make your stay as comfortable as possible, with the finest materials imported from all over the world and many aesthetic nuances, which adorn the hotel like jewellery. Accommodation: The ambience and hospitality at Kohinoor Continental match international standards. Each and every one of our 120 rooms is equipped with state-of-the-art lighting and furnishings. The well being of our guests is our main concern. So if theres anything you need at any hour, well get in for you. Be it access to the Internet or a bowl of fresh fruit, no desire of yours is too big or too small. Restaurant: A royal feast awaits your taste buds at Solitaire, the multi-cuisine restaurant at the Kohinoor Continental. Conference and Banquet facilities: With the unique mood lighting in our banquet hall, were sure your parties and conferences here will be evenings to remember. Indeed we had been closed for long but now you might be tempted to stay a little longer in the Kohinoor Continental. We look forward to seeing you soon. Recreation: If you want to unwind after a tiring day, then just melt in our pool or savor a Daiquiri at our bar. Health Fitness Centre: In case you want to work up an appetite then the Health Club and the sauna are just the places for you. The hotel incorporates environmentally friendly measures such as medium density fibre wood for construction materials, and plumbing, electrical, and mechanical systems that conserve water and energy This hotel offers 92 guestrooms and 14 suites in a six-story building. Air-conditioned rooms also come with ceiling fans. Among the amenities are coffee/tea makers, refrigerators, and wireless Internet access. Furnishings are made from environmentally friendly materials. 1.3 What is quality? â€Å"Quality itself has been defined as fundamentally relational: ‘Quality is the ongoing process of building and sustaining relationships by assessing, anticipating, and fulfilling stated and implied needs.'(Richard e. and Daniel k, 1996) As per the ISO quality is providing excellent products and services that all about meeting customer requirements. Its all about meeting the needs and expectations of customers. So a quality product orservice is one that meets the needsandexpectations ofcustomers. (Anon, 1994) Quality defined by as, conformance to the requirements which is the company itself has established for its product based directly on its customers need. (Crosby p,1979) 1.4 What is Quality audit? It has been a long established practice for major industries and sector to carry out on site evaluation of their most important and sub contractors. The quality audit undoubtedly began with customer inspection. These have been typically intended to : To prove technical capability of the establishment the product or service To check that establishment have capacity or not to cope the customer demand To confirm the commercial stability of establishment. To form an opinion as to whether this is the type of establishment that we would like to do business with. 1.5 Why is it necessary? It is a management tool covering services, products, equipment and financial aspect of a property. Most guests have no time to give feedback, or give only negative comments on one aspect of their experience. It measures and ensures service standard consistency within a chain/group. It identifies staff training needs and rewards. It identifies cost cutting efficiencies. Used for long term benchmarking data. 2.0 Quality audit for Kohinoor continental How you conduct the quality audit? Presently I am not working in hospitality industry therefore I chose Kohinoor continental for my quality audit. As I completed my training from there, so its easy to me to do the quality audit of Kohinoor Continental. In Kohinoor I worked most in bar therefore I chose bar section. Name of the bar Baryl club No of covers- 44   Ã‚  Ã‚  Ã‚  Ã‚  For quality audit purpose I have called to Bar manager and bar tender and asked several question regarding quality audit. 3.0 The gaps in Bar of Kohinoor Continental Lack of upselling The service personnel dont have any knowledge about upselling. Product out of stock- Sometime due negligence of steward, bar tender forgot to order the items which are low in stock. Therefore sometime we have to tell to our guest stock out of stock. No proper crowd management- Actually Baryl club offering heavy discount to there customer therefore there was very rush in the evening time. and its has only capacity of 40 cover at a time therefore crowd just roaming in the lobby and there is no one to handle that crowd from bar. Also there is no one for advanced booking. No proper storage area and very small pantry- In Baryl club pantry itself in the bar where we stored snacks (peanuts, wafers) some bottles of wine, beer, whisky etc. Also in pantry there is account department for bar section so its very congested to work. The pantry area is so small only two person can stand properly therefore its very difficult to work in peak hours. Less staff in bar- Although Baryl club has capacity for 40 covers only 2 peoples were there to serve them. Also there is no one to take the advanced bookings. Its difficult for staff to take the order and place the order and also take care the new guest. Also kitchen area is very far from bar section therefore if one bar personnel was busy to pick up the order from kitchen its very difficult for other to mange in the bar. I completed my training in bar therefore I observed these gaps during my training period. 4.0 Recommendation I am planning to fill the above gaps with PDSA model which is commonly used in hospitality industry. (Wiseman B. 2005) PLAN- it is the test how you are going to work. DO-how you are going to carry out the plan STUDY-look what are the result, what can you improve, what are your mistake. ACT- It is the designs time what are you going to do. 4.1 Recommendations on above gaps: Upselling- Recommendation for this to give your staff proper training for upselling. So they can also know what benefit of upselling is and also help them to earn some tips. Bar manager can also show some upselling practically so they can understand easily. Product out of stock-Recommendation for this is Bar manager have to keep eye on stock list so they can also order for stock without intimation of steward. Also Bar manager can check stock list in every 15 days so he is also aware about the stock in bar. Crowd management- Recommendation for this management H.R department have to hire a service personnel or public relation officer for bar so he/she can handle the crowd properly and also take the bookings on phone. Also management can keep separate area in lobby for those people who are waiting for table inn the bar. So they can wait in that area without disturbing front office staff. Storage area- Recommendation for this will be management can shift account department in the restaurant (where they already have account dept for restaurant) from bar and keep the that area fully utilised for bar purpose so that more stock can stacked properly and also no complaint about the lack of stock. After that during the billing we can give all KOTs to cashier in the restaurant and he can give us the proper bill and at the end of the shift we can handover the cash and all bills to cashier. Less staff in bar-Its very difficult to handle bar with only 2 staff therefore recommendation for this is management can recruit 1 or 2 more staff so its very easy to handle during the rush hours. 4.2 Action plan as per the recommendation and time spam Upselling-For upselling management can give proper training to food and beverage staff. The bar manager or the senior person can give proper training because of there experience. The training can held in the afternoon time when there is less rush or in the morning time when there is no guest in the bar. The manager have to show the upselling tactics practically so that they can understand it very easily and the will also try do upsellings. The training is for only once or twice in a week for a month. The one month period is enough to learn the upselling. Product of out of stock- Actually this is bar tenders duty to keep eye on the stock list. But due to negligence of bar tender some time products are of of stock. The solution for this is bar manager can check the stock list once in a fifteen days and he can also give the intimation to bartender about the stock. Another plan is they can save the stock list in computer and check the stock whenever the want and order as per the convenient. Crowd management-To fill this gap the H.R department have to appoint a public relation officer (P.R) for bar who can take the advance booking for bar and also control the crowd during the rush hours in bar. To appoint the P.R 15-20 days are enough after giving advertisement in newspaper or in job searching site. The P.R will also help in the bar to fill the tent cards asking the guest about the service and other things also like quality of food etc. Storage area- To fulfil this gap management have to shift the account dept of bar in the restaurant where they will see the accounts of restaurant as well as bar. The restaurant already have accounts therefore it doesnt take time for shifting purpose. But it will take 1 week to settle down there. After that in bar management have to make some storage shelves and proper area to stack the all items therefore this also take 1week time. Less staff in bar- For this gap H.R department have to recruit a steward or one more bar tender who will help service personnel from the bar. This will also take 15-20 days to recruit. One more option for H.R is they can hire the steward on temporary basis during the rush hours of the bar instead of hiring a permanent staff. For example they can hire a student form a hotel management college to work in the evening time. so it will save the cost as compare to hiring the a permanent staff. 4.3 Cost factor Upselling- As bar manager is giving the training during his duty hours so it doesnt cost to organisation. Product out of stock- This is covered in duty activity therefore this also doesnt cost anything. Crowd management- This will cost for organisation to pay the P.R staff. Storage area- This will cost only less amount to make proper shelves in storage area. Less staff in bar- This will cost as hiring of new personnel. 5.0 Bibliography Richard E. and Daniel K.(1996). Organisational orientation. Reference to: http://www.qualitydigest.com/html/qualitydef.html. (Accessed on 22 June 2008) Crosby P.(1979). Mini tutorial quality functional deployment. Reference to: http://66.102.9.104/search?q=cache:cxe5V4NPKRcJ:www.freequality.org/sites/www_freequality_org/Documents/knowledge/QFD.pdf+quality+definition+by+diffrent+authorhl=enct=clnkcd=1gl=in.. (Accessed on 22 June 2008) Reference to:http//www.praxiom.com/iso-definition.htm#Quality. (Accessed on 22 June, 2008)

Government Taxation Essay -- Taxes

There are two things in life that are certain: death and taxes. In today's world, the majority of our government's income comes from taxation. A tax is not a voluntary payment or donation, but an enforced contribution imposed by government (Mikesell, 2011). Taxes are an amount of money collected from citizens, and they are used to provide public goods and services to benefit our communities. Taxes are amounts established in a political process of structured laws to determine how the collective cost of government services will be distributed among elements of the market economy. The two most important tax policies are the level of taxation, or how much taxes should be, and the structure of the system, or how revenue is to be raised (Mikesell, 2011). To better understand taxation and its purposes, this paper will discuss in detail the different tax structures- income taxes, sales taxes and property taxes and describe their advantages and disadvantages based on equity, economic ef fects, collectability, and transparency. In the United States, taxes have been around even before the nation was founded. The criterion for taxes was proposed in a 1776 guide of taxation for a market-based economy by Adam Smith in the Wealth of Nations (Mikesell, 2011). During the Civil War, the government relied on excise taxes but they didn’t generate sufficient revenue, so the first income tax was enacted in 1861. By 1895, the tax was ruled unconstitutional by the Supreme Court, and in 1913 the 16th Amendment was passed which gave the United States the power to tax personal income and resolved the revenue problem (Mikesell, 2011). During the Great Depression in the 1930s, the government added unemployment compensation and social security progr... ...l never be levied fairly because the world is turning into the â€Å"haves† and the â€Å"have-nots†. Works Cited Davidson, C. (2006, July 24). The history of taxes. In Yahoo Inc. Retrieved November 23, 2010, from http://www.associatedcontent.com/article/45350/the_history_of_taxes.html?cat=37 Ellis-Christensen, T. (2010, September 10). What is retail sales tax? In Wise Geek. Retrieved November 21, 2010, from http://www.wisegeek.com/what-is-retail-sales-tax.htm Holetzky, S. (2010, September 26). What is property tax? In Wise Geek. Retrieved November 23, 2010, from http://www.wisegeek.com/what-is-property-tax.htm Mikesell, J. (2011). Fiscal administration: Analysis and applications for the public sector (8th ed.). Boston Pollick, M. (2010, August 2). What is income tax? In Wise Geek. Retrieved November 21, 2010, from http://www.wisegeek.com/what-is-income-tax.htm

Custodial issues

Every year thousands of prisoners are released from prisons on parole or after completing their jail terms. To ensure that prisoners return to their normal lives without re- offending governments adopt programs meant to prepare prisoners for life after prison. Such programs may involve prisoners being allowed to work in community projects in order to instill a sense of responsibility in them. Additionally, prisoners may be allowed to work away from prisons and return when they have finished work. In some cases prisoners are permitted to visit the community.But despite these noble initiatives, cases of prisoners re-offending soon after they are released from prisons are not uncommon. This reveals that the programs are not always effective and something needs to be done to improve the situation. I strongly agree with probation experts such as Maburi (2009) that the psychological confusion, social stigma and economic hardships that they experience are partly to blame for prisoners re-of fending tendencies. I believe that the excessively punitive sentences issued to convicts harden them instead of reforming them.Such sentences ignore the fact that convicts will at one time return to the community yet and do little to prepare them for life after prison. To prevent cases of ex convicts re-offending, courts should take bear in mind rehabilitation of prisoners back to the community after their jail terms. Towards this end, I believe there is need for further cooperation between the judiciary and the prisons and rehabilitation departments. By issuing humane punishments, courts will not only help in decongesting prisons but also save time and resources spent in charging and convicting the same persons for the same criminal offence repeatedly.This will definitely boost efficiency of both departments. While the move by governments to rehabilitate ex-prisoners into the community is laudable, I think there is need to enhance cooperation between rehabilitation departments and prisoners’ families and communities. This helps reduce stigmatization of prisoners thereby helping them regain their self esteem and quicken their reintegration into the community. The community also benefits from reduced cases of crimes. At the same time, by working closely with ex convicts, community leaders are able to monitor ex convicts movements and detect any criminal tendencies.Such monitoring also protects reformed ex convicts from being accused of criminal behaviors. I also believe that there is need to increase financial assistance accorded to ex-prisoners. As a result of social stigma that faces ex convicts, they face challenges in securing employment, consequently some of them may resort to criminal activities such as robbery to meet their needs. To reduce chances of ex prisoners re-offending, I would strongly advocate for the creation of organizations based on the alcohol anonymous model. This would entail prisoners and ex prisoners coming together to help each other overcome their criminal behaviours.Since they share similar experiences it would be easier for them to open up to each other and get assistance. Such a move will help reduce recidivism and other offences. Finally, I would propose that ex prisoners be made to pay back to the community for crimes committed. This could help deter them from committing crimes and boost reconciliation efforts in the community. References Maburi T, K (2009) Re-Integration of Ex-Prisoners Back Into Society – What More Can We Do? In The New Times 26 October 2009 retrieved from http://allafrica. com/stories/200910270086. html on May 10, 2010

Internal And External Factors Effect The Levels Of Management Essays

Internal And External Factors Effect The Levels Of Management Essays Internal And External Factors Effect The Levels Of Management Essay Internal And External Factors Effect The Levels Of Management Essay How Internal And External Factors Effect The Levels Of Management For a company to be successful in the long term today. they must be diverse. The company needs to be able to accommodate to alterations. The demand for these alterations can show themselves from many angles. Some would include alterations in the economic system. selling. or even a alteration in the mark audience. A company has to look at more than merely the four degrees of direction. Organizations must look at how internal and external factors can impact the four degrees of direction. and adjust consequently for these alterations to be successful in today’s universe. Diverseness There are many types of diverseness. racial. cultural. cultural. age. gender. and geographic. But diverseness can besides include experience and accomplishments. A company’s ability to last depends on their ability to accommodate to current alterations. The diverseness within a company must be taken into consideration during the four maps of direction. The most successful companies will be those that recognize the power of diverseness in their work force and in the merchandise mix. and efficaciously create merchandises and services that appeal to their progressively diverse client base. By including diverseness in the planning phase. Amazon. om would reflect diverseness in their concern. This would consequences in better merchandises and a wider scope of consumers giving them an advantage over rivals. The company must understand its’ organisational construction. and the societal and cultural positions of its’ employees in order to be effectual. efficient. and advance an environment suitable for advancement. Employees who are diverse in accomplishments. experience. and background aid to construct a strong organisational foundation. Increasing globalisation requires companies to hold more interaction with diverse civilizations and backgrounds. Organizations must be prepared to learn themselves and their employees to value multicultural differences. Management must actuate their employees to go to diverseness preparation in order to be educated on different civilizations and traditions and to esteem these differences. Diversity is critical to a company’s success. We are portion of a world-wide economic system with planetary competition. Diverseness can increase productiveness. selling chances. enlisting. and the organization’s image which. in bend. can increase net incomes ( Leading With Diversity. neodymium ) . Globalization Globalization has changed the face of concern. In today’s concern market. international concern is necessary in order to derive an advantage. Amazon. com understands this demand. With the formation of the Internet and Internet selling. globalisation was a good fringe benefit which changed the concern market. supplying a manner for companies to make concerns and clients from around the Earth at the chink of a mouse. Amazon. com was created to work in the planetary concern environment through the Internet. The company handles B2B and B2C dealingss and minutess. Amazon. com uses an on-line client informations base to maintain up with the demands of clients. The company created an Internet e-business theoretical account. and built up from these based on information obtained from clients. The company’s strong substructure supports its mission to be the leader in e-commerce. ( A Conceptual Analysis f the Key success of Business in terms of Internet Marketing. 2012 ) . Customers are ever looking for convenience in today’s planetary economic system. Amazon. com uses technological invention to drive growing and present convenience to its clients. An online concern. such as Amazon. com. bases ends for Planning on these factors. In order to present strategic value. a company such as Amazon. com works to widen globally to make clients and retail merchants internationally. Amazon. com began with a B2C platform. but direction saw the possible to be an on-line planetary leader by managing B2B dealingss every bit good. When Forming. direction adds package. e-commerce capablenesss. and entree for concerns to utilize the online company’s resources. Amazon. com has created a planetary online concern through proper planning and forming that delivers strategic value. Management Leading can mobilise and actuate employees. Amazon. com has extended its gross revenues and merchandises through drawn-out subdivisions in different parts of the universe to supply services anytime and anyplace through the Internet and globalisation. Through the Controlling procedure of direction. online companies such as Amazon. com have continuously improved and expanded services to all over the universe. As a consequence of success through right using the four maps of direction. Amazon. com is a taking luck 500 company who uses globalisation through the World Wide Web to present fast. successful services to clients and retail merchants. Without the possibility of globalisation through engineering. e-commerce would non be plenty for the company to remain on top. Technology Technology is invariably altering and well hard to maintain up with. Because of these alterations. pull offing at Amazon. com would decidedly be a challenge. Directors must get down with the planning stage by measuring and finding consumer’s demands for purchasing. merchandising. utilizing. and transporting which can demo both internal and external growing. Personal information is shared online and is protected to guarantee safe minutess. Amazon. com ensures that individuality larceny is neer an option through their engineering. Forming all the informations gathered from engineering can besides assist direction maintain path of tendencies. gross revenues. and possible returns. Once this information is shared among direction. they are so able to take employees with preparation on how to implement new engineering like package. security protection on waiters. Amazon. com besides ensures employees are adhering to company policy with the usage and entree of services online. The commanding stage of direction is completed by watching over the advancement of smart phone applications. Internet place usage. and transporting gross revenues. By watching advancement and being certain initial ends and challenges are being met. the security of consumer information and merchandises are non being put to put on the line. and if a job arises. direction can catch it early plenty to take attention of the state of affairs. With engineering doing entree to Amazon. com so much easier for consumers. the direction of this changeless altering engineering can be a immense challenge with so many options for entree and maintaining the information protected with encodings. Invention With an of all time altering market. a great concern has to be able to go what the consumer demands at any given minute. This requires invention. Innovation involves any new thought. merchandise. procedure. engineering or service that a concern introduces to its consumer. When it comes to invention and the planning procedure. a concern needs to be cognizant that factors such as the market. the customer’s current demands. and even what merchandises rivals have released. can impact the current concern program. There needs to be flexibleness in the concern program to account for these alterations. A concern demands to hold leaders who are able to understand how their concern is being presently run and how it can be run in the hereafter. This allows them to see ow alterations in the concern program might impact all facets of the concern. A concern director needs to be open-minded and promote its employees to portion their thoughts on new concern theoretical accounts. new ways to do a net income and even ways to better bing concern methods so that the current concern program is both advanced and effectual. When it comes to invention and the forming procedure. a company needs to be cognizant of the current concern program at all times. The demand for a concern to be advanced and adapt to the alterations in the market can do all four maps of direction to necessitate seting often. If the concern program is changed. the company needs to re-gather and delegate all its resources. employees and fundss to guarantee that the new program can be implemented to the full and in an organized mode. Invention affects this map of direction in a more indirect manner ; by altering the planning procedure. the directing procedure alterations. When a company decides to alter their concern program. they have to re-direct the staff. When measuring the staff. the director needs to be certain that they are on path to run into the ends outlined in the planning procedure. Invention can do the ends to alter. therefore doing directors to re-evaluate the employees to be certain they are still on path. Ethical motives Every concern is run by a set codification of moralss. Ethical motives are different for every individual. as they are an individual’s personal return on what is right and what is incorrect. A concern uses a codification of moralss to guarantee that every employee abides by the same set of criterions and upholds the values of the organisation with which they work. When it comes to the planning procedure. moralss play a cardinal function. The company needs to cognize how they want their concern to look to the populace and develop their program around that. If a company wants to look ethical and trusty to its clients it has to supply great client service at all times. This meant that Amazon. com had to make a safe site. a choice merchandise. and be certain to present the merchandise quickly. When it comes to forming and moralss. Amazon. com had to be certain to maintain all resources. employees. and fundss in order to maintain their word on the quality of both their merchandise and their bringing times. For the client to believe that Amazon. com is an ethical and trusty site requires employees and resources to be organized at all times. When it comes to taking their employees. it is of import to be certain that they are non merely on undertaking but besides following the company’s codification of moralss. If holding a safe site. a choice merchandise. and prompt bringing is of import to Amazon. com ; it needs to be of import to all their employees every bit good. When analysing the advancement being made. the director needs to besides be certain that the staff is staying diligent in their attempts to guarantee quality client service. Ethical motives are found in every aspect of a company and therefore they need to be monitored on a regular basis. Decision There is so much more that goes into keeping a successful concern in today’s competitory market place than merely the four degrees of direction. The company must ever be cognizant of and be able to accommodate to many altering factors. These factors come from many topographic points in the market place. Some are external and the company will hold small to no control over these and must merely accommodate. However the internal factors can be controlled by the company through monitoring and policy alterations. The company that can detect the changing factors and integrate and adapt to these alterations will be successful in today’s competitory market place.

HOPE Foundation essays

HOPE Foundation essays In Georgia, there is a program going on that helps out the student immensely. The Georgia State Lottery is used as a device to send hard-working students to college for free. Since the start of the lottery, over 488,00 kids have gone to college on the HOPE scholarship due to the lottery (Message from the President 1). Also, the lottery helps pay for pre-kindergarten facilities and programs, as well as computer and technology upgrades for their high schools through the HOPE foundation. North Carolina needs to take a serious look at the revenue Georgia brings from its lottery and think how it could be beneficial for North Carolina students. First, the lottery helps out a tremendous amount of students through the HOPE program. The way that the Hope scholarship works is that all Georgia high school students that graduates with a 'B' average or higher becomes eligible to receive a grant to help cover the cost of tuition, book, and mandatory fees at any public Georgia college or university and a $3,000 dollar scholarship to any private college (Educational Uses of Lottery Proceeds 1). Also, they will provide full tuition, books, and fees to any technical institute in the state of Georgia. That sounds like a whole lot of incentive for young adults to make a 'B' average in school. I just don't understand why North Carolina does not have the same incentive for our children. To keep the college student a good student, you must also keep a 'B' average in college to keep your scholarship. Georgia has given more than 1 billion dollars toward college bound students since 1993 (HOPE scholarship 1). North Carolina has no pro grams of this sort. Two years after starting college, HOPE recipients have a higher G.P.A. (grade point average), and have earned more credit hours than their counterparts who do not have the HOPE scholarship according to The Council For School Performance (Council for School Perfo ...

Demand and Supply in Organization Success Research Paper - 14

Demand and Supply in Organization Success - Research Paper Example Thus by understanding the consumer demands, the organization can identify their desires and will only stock the commodities that provide utility to the users. Hence, the organization will have a wider knowledge of what the demands are relative to what the supplier can offer at a particular market rate (Bustinza, Parry, & Vendrell-Herrero, 2013). Thus, prices and availability of goods and services are predetermined to the consumer. This ensures that customers do not miss out on their wants whenever they come to purchase a particular commodity. Thus, trust and loyalty are built between the two parties that will result in more sales hence increased profits to the firm. Rain checks are forms of compensations that are usually offered to consumers when the goods and services they had ordered are unavailable majorly due to the organization being out of stock or late deliveries. Customers are thus promised the assistance to acquire the products at prices indicated earlier and did not have to spend more regardless of any economic crisis like inflation. Customers are thus assured to get their services or be compensated and will not encounter any losses that create a mutual trust for both parties. However, to say this is a fair treatment of consumers is understatement due to economic injustice it creates to the consumers. Individuals usually derive their satisfaction at a particular time and have more tastes to it is provided during that period rather than a later date. Thus, postponement of the consummation of a particular good or service may lower the satisfaction of products to customers and reducing their utility.

Knowledge Management and Innovation Essay Example | Topics and Well Written Essays - 2000 words

Knowledge Management and Innovation - Essay Example 855). This piece of research work critically examines the knowledge sharing theoretical models developed by Nonaka and Takeuchi to elucidate how this model has been implemented in a typical industry to apply KM to the innovation of a product. This paper compares and contrasts knowledge transfer models based on relevant literatures and empirical evidences. Knowledge Management and Innovation In the contemporary business contexts where markets, products, competition, technology, laws and consumers are rapidly changing, continuous innovation and effective practice of KM to support innovation has become a major source of competitive advantage (Aramburu, Saenz and Rivera, 2006, p. 157). Nonaka and Takeuchi (2005, p. 1-5), both are widely renowned as KM gurus, argued that Japanese companies remained as enigma for most Western business experts and they gave a new explanation of what made Japanese companies highly successful. ... ased on this explanation, the three major components of KM are creating the knowledge, sharing it and using the created or shared knowledge for organizational purposes. Some literatures have considered more components such as knowledge identification, knowledge collection, knowledge organizing, knowledge dissemination, knowledge evaluation and knowledge adoption or implementation (Mehrabani and Shajari, 2012, p. 166). Forster (2005) described that knowledge management is a generic term that includes certain process by which employees’ experience, knowledge, skills etc are gathered, shared and utilized and then converted to a system of collective organizational learning so as to improve organizational performance. Nonaka and Takeuchi perceived knowledge as a profound instrument that has effectually increased organization’s capability to implement right action from the evidences of Japanese companies. Knowledge has been categorized in to two; tacit and explicit knowledge. Tacit knowledge is one that is embedded in a person through the forms of experience, knowledge, skill, talent, wisdom etc and this can be attained from imitation and practice. Explicit knowledge is information stored in documents or database (Lee, Leong, Hew and Ooi, 2013, p. 850). Literatures in the field of KM have clearly illustrated the profound impact of knowledge management on innovation. Most firms in contemporary business contexts find innovation as their grand strategy due to that consumers expect regular changes and advances in products (Pearce and Robinson, 2004, p. 207). Innovation is not merely an idea, but rather, it is the conversion of idea in to business functionality by establishing the idea in to reality (Endsley, 2010, p. 1). Many literatures including Boghani (2007), Lee et al

What are the ultimate principles of morals for both Hume and Kant Essay

What are the ultimate principles of morals for both Hume and Kant - Essay Example He goes on to observe that only good will is good sans qualification. Good will is always good in itself and not just for the things that it produces. Will is good if will comes from duty, as well as other moral motives, which do not simply conform to duty. For instance, grocers who give the right change because of fairness, rather than from the fear of being caught, do so from good will. The reasoning goal is not in producing happiness but rather producing will that can be seen to be good in it. The satisfaction of man’s desires, which is happiness, is not determinate enough to use as a workable guide. Good will cannot act as the complete and sole good, although it is the worthy condition of being happy and the highest good. Complete good can only be goodwill combined with happiness. Rationality possesses its own laws of objectiveness. Because man is rational only partially, he experiences the various laws as constraints and imperatives that he is required to follow. These im peratives are grounded on the premise that is valid for all rational beings as such. These imperatives, according to Kant, could be hypothetical, i.e. if one wants to get to end E, then he should do A, or categorical, which is he ought to perform A. Ethics that are based on imperatives that are hypothetical are heteronymous because they involve following laws set by another. Categorical imperatives are hard to understand, although their content is clear. The basic imperative of category states that man acts on principle and these principles can be willed on everybody (Laursen 21). Ethics and morals are autonomous if they are based on categorical imperatives because man follows their own laws. Kant’s supreme moral principle is the universal law formula, which contends that man should act on a maxim that they follow to be used as a universal law (Laursen 23). Kant continues by applying his formula to two duties that are perfect and exception-less. These are not making promises with deceit and not committing suicide, as well as two imperfect duties, which to help those who have needs and development of one’s talents. These ideas can be expressed more loosely in two ways. Firstly, it is to treat all humanity, both you and others, not as a means only, but also as an end in itself (Laursen 23). Secondly, man should act as if one’s action maxim would be turned into nature’s universal law. Kant also connects freedom with morality. According to him, to be free means to follow one’s own principles of rationality rather than following our desires only. This means that one should follow their own legislation and act on maxims that they would follow, rather than the universal laws (Laursen 24). Therefore, freedom is morality. So morality and freedom, ultimately, are the same mystery. It is not possible to explain what free will is. It is only possible to assume what it is and reject all objections against it. Man knows that he/she is free through knowledge of his/her duties. Since probably an individual could have acted in a different way, then one is free. In order for man to recognize himself as free, he needs to see himself as being a member to two worlds. These worlds are a higher intelligible world and a sensible world. Acting in a moral way, therefore, has a supreme moral worth since through it, one participates in an existence of a higher order. This can be perceived as the foundation of human dignity as an end to itself (Laursen 24). Hume, on the other hand, contends that reason and moral sense combine to make our moral judgments. He says that moral sense is essential in making a distinction between virtue and vice,

Nurse Anesthetists Essay Example for Free

Nurse Anesthetists Essay In a career in nursing, one must possess a need to help people, as well as the scientific and educational knowledge to perform the duties required. As I complete the final years of my high school education, I realize that I must focus on my goals and interests in order to make intelligent college curriculum decisions. I have always been considering a career in health, but the opportunities and diversification in the health field have perplexed me. I have always thought the job of a nurse anesthetist was extremely interesting, and it would be a great way to contribute to helping the lives of others. In the United States, a Certified Registered Nurse Anesthetist (CRNA) is simply an advanced practice registered nurse (APRN) who has acquired graduate-level education and board certification in anesthesia and they are the oldest nurse specialty group in the U. S. However, their job isn’t that simple. Anesthesia describes drugs and gases that help to block sensation, and they keep patients unconscious while in surgery. The main goal of the CRNA (Certified Registered Nurse Anesthetist), which is extremely important, is to have the patient awake free of pain. CRNAs, or Nurse Anesthetists, are responsible for administering, supervising, and monitoring anesthesia related care for those patients undergoing surgical procedures. Before the surgery, the CRNA obtains information regarding the patient’s medical history, evaluates the patient’s anesthesia needs, and develops a treatment plan with the goal of a risk-free and uncomplicated surgery. CRNA then explains the planned procedure to the patient, and answers any question that they may have. However, there are big steps that you have to take to become a CRNA. According to the American Association of Nurse Anesthetists (AANA), here is what you need to do to become a nurse anesthetist. All would-be nurse anesthetists must have an active registered nurse (RN) license, which requires a minimum of two years of study. Applicants to nurse anesthetist programs should have at least one year of work experience as an RN working in acute care, such as an intensive care unit. Once admitted to the Anesthesia program, the registered nurse must complete twenty-four to thirty-six months of classroom and clinical experience. A bachelors degree is the minimum required educational credential for certification as a nurse anesthetist. Many training programs are structured as masters degree programs, which always require a bachelors degree. Accredited Training Programs is the most important requirement to become a nurse anesthetist. Different programs have different specific requirements for application. Upon completion of the training program, prospective nurse anesthetists need to pass the certification examination offered by the Counsel on Certification of Nurse Anesthetists, and then maintain certification by regularly taking continuing educational units. Nurse anesthetists must obtain the appropriate licenses to practice in their state by contacting the state board of nursing for more information.

Pseudomonas Aeruginosa Gene Expression

Pseudomonas Aeruginosa Gene Expression Pseudomonas aeruginosa is the gram negative rod shaped bacteria is found in all natural and man made environments. the organism is so stubburn so that it can grow in any environment and also at high temperatures such as 420C. The unique ability of the organism is forming biofilm. The biofilms are formed by the organism with the association with a surface. The surface may be anything such as cloth, paper, glass etc and any artificial surface. It is the opportunistic pathogen which behaves neutral to the normal tissues but it infects once the defences of them are compromised. The development of the biofilm starts with the attachment of the freely moving planktonic cell to a surface. Generally the twitching motility of the P.aeruginosa helps to use its unipolar flagellum to adhere a surface. The bacterium develops colonies known as microcolonies and adhere to the surface strongly which are known as pili structures (Baron S et al.,1996). During the biofilm development several factors pla ys a key role mainly the exopolymeric matrix. The EPS matrix is not visualised directly, but it can be done by using some fluorescent dyes. The functioning of the EPS matrix in holding cells of Pseudomonas aeruginosa together and forming the mature biofilms. The biofilm develops with cell division and recruitment. The cells interacts via quorum sensing and these interactions are studied under scanning electron microscopy. The biofilm formation takes place in stages and many changes are observed in its size and shape during the development. The EPS matrix is made up of polysaccharides, proteins and nucleic acids. The exopolysaccharide matrix is the important component of the biofilm. The exopolysaccharide genes such as pilB, pslA, lecA, ureB and alginate genes are responsible for the formation of pseudomonas biofilms. The locus of the psl polysacchide genes encode the cells to adhere to a surface and thus maintaining the biofilm structure. In this research project we try to quantify the gene expression of the above genes pilB, pslA, lecA, ureB using rpoD as the house keeping gene. The biofilms are grow invitro on microcarriers. The microcarriers made up of dextrons in the range of 60-90 µm in size are used as a surface for Pseudomonas aeruginosa to form biofilms. The microcarriers are spherical in size where the bacteria can easily adhere on surface. In this present work we try to grow planktonic cultures and biofilms cultures on the microcarriers in a fermentor separately. The genes responsible for the biofilm formation are quantified by comparing the planktonic and biofilm samples. The planktonic cells grow very rapidly whereas the bacterial adhering takes some time to form biofilm in other case. The samples are collected at regular intervals and mRNA is extracted. The gene expression is quantified using real time PCR. The relative quantification method of qPCR or real time PCR method does not require any standard curve for the analysis where the samples are normalised by using an internal control. The threshold CT values obtained from the curve are used to calculate relative quantification by comparative à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  CT method. The results from the planktonic and biofilm gene expression results are compared. On the other hand we also assay the nutrients such as glucose, ammonia, phosphorus and protease during the fermentation. The concentration levels of the nutrients are also compared for both biofilm and planktonic fermentation. Review of literature: Pseudomonas aeruginosa is a member of gamma proteobacteria class of bacteria. The bacteria is gram negative, rod shaped and aerobic belongs to the bacterial family of pseudomonadanceae. The recent developments in the taxonomy based on the 16s ribosomal RNA or conserved macromolecules, the family members are divided into eight groups. Pseudomonas aeruginosa is a species present in this group sharing the with other twelve members. Pseudomonas aeruginosa is a free-living bacterium, commonly found in soil, water, skin flora and in all man made environments. The organism regularly occurs on the surfaces of the plants and animals. Pseudomonas aeruginosa is an occasional pathogen to plants. It is also an increasingly emerging pathogen of clinical relevance. The organism not thrives in the normal atmosphere but also in the places with little oxygen and thus forms the colonies in natural and artificial environments. Pseudomonas aeruginosa is an opportunistic pathogen for humans, which means that it exploits some break in the host defences to initiate an infection. The bacteria never infects the uncompromised tissues, but it can infect any if the tissue defences are comprised. It can cause infections such as urinary tract, respiratory system infection, soft tissue infection dermatitis, gastro intestinal infections. It causes a variety of systemic infections in patients particularly with severe burns and immune suppressed in cases of cancer, AIDS, cystic fibrosis in lungs. Characteristics Pseudomonas aeruginosa is rod shaped gram negative bacteria. The size of it ranges between 0.5 to 0.8  µm by 1.5 to 3.0  µm. almost all the strains of Pseudomonas are motile with a unipolar flagellum. It is ubiquitous in soil ,water and on the surfaces in contact with water or soil. It has respiratory metabolism and can grow in the absence of oxygen if NO3 is available as respiratory acceptor for electron. Generally the bacterium is found in nature in the form of biofilm attached to a surface or substrate, or in the planktonic form as a unicellular organism swimming actively with its unipolar flagellum. It is one of the most vigorous and fast swimming organism found in pond waters. It can grow at temperatures at 42OC or high, but the optimum temperature is 30 to 37OC. it is resistible to a wide variety of physical conditions such as temperature and can even resist the salts, dyes, antiseptics and most commonly used antibiotics. Pseudomonas aeruginosa produces different types of colonies from the source they have isolated. They appear like small and rough colonies. Moreover the samples collected from the clinical sources look like a fried egg like structure which is smooth, large with elevated appearance. The other type of colonies obtained from the respiratory and urinary tract secretions looks like mucoid.this mucoid appearance is attributes for the production of alginate slime. These smooth, mucous colonies presumably plays an important part in the colonization and virulence. Pseudomonas aeruginosa produces two types of soluble pigments. Pyocyanin which is a blue-green and a yellow-green and fluorescent pigment called as pyoverdin. Pyocyanin is produced abundantly in the media with low levels of iron and also functions for the iron metabolism of the organism. Many strains of Pseudomonas aeruginosa produces pyocyanin but not all the strains does it. Although the organism is classified as the aerobic, sometimes the organism is considered as facultative anaerobe where it can adapt and proliferate the conditions with less or total O2 depletion. The organism can grow in the absence of oxygen if NO3 is available as respiratory acceptor for electron. It can ferment arginine by substrate level phosphorylation where the layers of alginate present surrounding the mucoid cells of the bacteria controls the diffusion of oxygen(Collins FM, 1955), this is witnessed in the cystic fibrosis of the lung infection. Pseudomonas aeruginosa which is found in the biofilm form is responsible for a variety of activities involved which are natural and artificial. Resistance to antibiotics Pseudomonas aeruginosa is opportunistic pathogen, has a notorious resistance to antibiotics. The organism is very resistance towards many antibiotics due to the potential permeability barrier provided by the gram negative membrane. Pseudomonas aeruginosa in the biofilm form makes the cells impervious to the concentrations of the therapeutic antibiotic. The organism gets associated with others like actinomycytes, moulds and bacillus because of the natural environment they live. Moreover the organism have antibiotic resistance plasmids R factors and RTFs, the genes are able transferred by conjugation and transduction following the mechanism horizontal gene transfer(HGT) (Todar.K,2008). Biofilms Biofilms are defined as a matrix enclosed bacterial populations which are adherent to each surfaces or interfaces. Microbial aggregates and floccules and also adherent populations within the pore spaces of the media are spoken in the definition (Costerton J.W, et al 1995).bacterial biofilms came into existence and became significant from their first recognition in their ubiquity. The past researches show that profoundly the growth phase of the biofilms is distinct from the planktonic. In response to the surfaces proximity, bacterial cells alter their phenotypes (Costerton J.W, et al, 1995). The early stages of biofilm formation the bacteria is in stable juxta position with those of same and other species. The micro environmental conditions, cellular juxtapositions and the extra cellular polysaccharide matrix constitute to the development of the bacterial biofilm. Different bacteria responds to the specific environmental conditions with certain growth patterns, and a structurally comp lex and mature biofilm develops(Hamilton W.A et al, 1987). Physiological cooperation is the major criteria in shaping structure and juxta positions establishment to make biofilms adherent for the surfaces. It has been proposed that large suites of genes are expressed differentially regulated. Formation The formation of the biofilm initially starts with the attachment of the freely floating bacterial cells to a surface. The bacterial colonies adhere to the surface with weak vander wall forces. These colonies can lead themselves to more adhesion called as pili structures (Baron S et al.,1996). The organism adheres more to the available surface by binary fission, exopolymeric matrix is produced to develop mature biofilms. The matrix produced holds the biofilm together. Biofilm develops with cell division and recruitment, the cell in the biofilm communicate via quorum sensing. We can observe changes in shape and size in the final stages of biofilm formation. Distribution and ubiquity of biofilms The general phenotypic strategy of organism is to change in the mode of growth that to adopt to the altering growth conditions. The cells of Pseudomonas aeruginosa which is a ubiquitous bacterial species respond to all favourable nutrient conditions. The organism adheres to the available surface by binary fission, exopolymeric matrix is produced to develop mature biofilms. The rod shaped bacteria grows in matrix which is enclosed in a sessile growth mode. The cells are protected from the biological, antibacterial, chemical agents and adverse environmental conditions. The periodically shed planktonic cells from the biofilm provide and sacrifice the protection to the new fresh habitats can colonize with the biofilm. The adherent biofilm populations has a significant metabolic activities( Fletcher.M, 1986). The extent of biofilm formation is predicted in the particular system is based on the following principles: Metabolically active bacteria show tendency towards the surfaces for adhesion in all natural environments. In all environmental conditions the extent of biofilm formation is controlled by the available nutrient for cell respiration and exopolysaccharide production. The organic nutrients sometimes tend to associate with the surfaces, in such cases the local biofilm is difficult to trigger. This is more in extremely oligotrophical environments, but bacteria normally does not adhere to surfaces in nutrient less environments. Pseudomonas aeruginosa Biofilms The communities of microbial bacteria(Pseudomonas aeruginosa) forms a definite structures with the association with surfaces are abundantly available environment. These structures are often called as biofilms which are less susceptible to antimicrobial agents. These biofilms causes chronic infections and very difficult to remove. The extracellular polymeric matrix(EPS) are known to holds the biofilm structure and it performs as a matrix. The EPS matrix holds the biofilm cells together and protects them from shear forces in fludic environments. The extra cellular matrix for Pseudomonas aeruginosa forms the biofilm development in different stages. The matrix holds the cells together on the surface and forms a critical structural design resists to many environmental changes. Pseudomonas aeruginosa biofilm develops in a five stage multicellular cycles, the process is initiated by the attachment of planktonic (free) cells to a surface. This is followed by the formation of microcolonies. T he microcolonies are seeded and gets dispersed where the cells form the microcolonies comes out to occupy a new surface( Ma. L et al, 2009;Stoodley.P et al,2002). The EPS of Pseudomonas aeruginosa composed of polysaccharides, proteins and nucleic acids. It is the important component of the biofilms. The exo polysaccharide genes lec A , Pil B, Psl A, alginate are responsible for the formation of Pseudomonas biofilms. The locus of the Psl exopolysaccharide encodes bacterial cells to surface and thus maintaining the biofilm structure. The matrix which is anchored by the Psl is not visualised directly during the developmental stages of Pseudomonas aeruginosa biofilm. In addition to the present scenario of exopolysaccharides, extracellular DNA also plays an important part in . P.aeruginosa biofilm matrix (Allensen-Holm M et al, 2006). The extracellular DNA is derived from the chromosomes and it functions for signalling between the cells interconnecting the biofilm matrix. In the biofilm matrix, extracellular cellular DNA promotes cation gradients, release of genomic DNA and induces antibiotic resistance ( Mulcahy H, et al. 2008). Real time PCR Polmerase chain reaction or simply PCR is introduced by Kary mulis in 1980, which really a revolution in the molecular biology. PCR is the standard procedure to amplify small quantities of DNA molecule into large quantities. The method depends on thermal cycling which involves the continuous heating and cooling of reaction for DNA melting and enzymatic replication of DNA. PCR applications employ a general heat stable DNA polymerase such as Taq polymerase enzyme isolated from the bacterium Thermus aquaticus. PCR method is involves three major steps.1) Denaturation 2) Annealing 3) Elongation. In the first step DNA template is denaturated to single strand at high temperature of 940C. In the second step, single stranded DNA template annealed with primers, the temperature is 650C. At 720 C the DNA starts elongation which is third step. DNA polymerase synthesizes new complementary strand to the template DNA strand. Real time polymerase chain reaction or qualitative polymerase chain reaction is a laboratory technique which not only amplifies but also quantifies the target DNA molecule. it helps the user for detection and quantification(absolute and relative) of many specific genes in a present in a given DNA sample. The general principle of polymerase chain reaction (PCR) is also used in the RT PCR. Unlike PCR, RT PCR is a new technology product is detected at the end of the reaction. There are two common methods for the product detection in RT PCR 1) Non -specific fluorescent dyes which interacts with the double stranded DNA molecule 2) Sequence specific DNA probes consists of oligonucleotides that are fluorescently labelled with reporter. The product is detected after the hybridization of the probe and complementary strand. The RT PCR is used to amplify the reversly transcribed cDNA products from the mRNA, which is becoming the effective and powerful tool in the field of molecular biology to quantify and study the gene expression. The RT PCR method is very easy to apply provides the reliable, accuracy and rapid gene expression quantification. The accurate nucleic acid quantification requires mathematical application for data analysis(Michael W.P., 2001). The real time PCR provides the very accurate and reproducible quantification of gene copies (Heid C.A., 1996). Unlike other PCR methods, RT PCR does not require post process sample handling, prevents product contamination, provides faster and accurate assay results. Real time PCR is very accurate and less labour usage than the present gene quantification methods. Nucleic acid sequence quantification has an important role in the biological research. Measuring the gene expression have been extensively used in the biological responses to various external s timuli( Tan et al, 1994; Huang et al,1995). Gene and genome quatification also used for HIV demonstrates the change in the expression level of virus in the disease period( Conner et al. 1993); (piatak et al, 1993). There are two types of quantification methods used in real time PCR: Absolute quantification Relative quantification Absolute quantification determines the input copy number generally by relating the PCR signal to a standard curve. In this method equal input amounts of sample, each sample under analysis amplifies with identical efficiency up to the point of qualitative analysis( Heid et al,1996). We quantitate the unknowns based on a known standard quantity. The unknown samples are compared to the known by creating a standard curve. The input copy number of the PCR signal is related to standard curve in absolute quantification method. Sometimes it is not necessary to know the copy number but it is important to calculate the relative change gene expression (Livak.K.J et al, 2001). The second method is relative quantification, we analyse the changes in gene expression in a given sample relative to another reference sample(untreated control sample). Relative standard again consists of two types of methods they are relative standard curve and comparative Ct method. In the relative quantification the PCR signal is related to the targeted transcript of the samples in the group. The new applications of comparative Ct method is the 2-à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  Ct method which is efficiently useful for the analysis of qPCR data. The 2-à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  Ct method(Livak.K.J et al , 2001): Derivation of 2-à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  Ct method: The equation for the amplification of PCR can be written as: Xn=X0 (1+Ex)n(1) Where Xn is the number of target molecules at cycle n of the reaction. X0 is the initial number opf target molecules. Ex is the efficiency of the target amplicon. n is the number of cycles CT is the threshold cycle, indicates the fractional cycle at which the amount of amplified target reaches a fixed threshold. XT =X0 (1+Ex)CT.X=Kx(2) Where XT is the threshold number of target molecules. CTX is the threshold cycle for target molecule and Kx is constant. A similar reaction for the internal control gene is RT =R0 (1+ER)CTR=KR-(3) Where RT is the threshold number of reference molecules R0 is the initial number of reference molecules ER is the efficiency of the reference molecule. CT.R is the threshold cycle for reference amplicon. KR is a constant. Dividing XT and RT, we get , (XT/ RT )= (X0 (1+Ex)CT.X) / (R0 (1+ER)CT.R )= (Kx / KR ) = K-(4) For real time amplification using Taqman probes the exact values of the XT and RT depend upon the number of factors including the reporter dye and fluorescence properties of the probes, efficiency and purity of the probe, florescence threshold setting. Thus k cannot be equal to one. Assume the efficiencies of target and the reference as equal. ER = Ex=E (X0 / R0) (1+ E) CT.X- CT.R =K(5) XN (1+E)-à ¢Ã‹â€ Ã¢â‚¬  CT =K-(6) Where XN is equal to the normalised amount of target gene (X0 / R0) and à ¢Ã‹â€ Ã¢â‚¬  CT is the difference of the threshold cycles of target and reference (CT.X- CT.R ). The expression can be written as XN=K(1+E) -à ¢Ã‹â€ Ã¢â‚¬  CT -(7) If we divide the whole equation by XN for any sample q by XN as the calibrator(cb) XN.q /XN.cb ={[ K(1+E) -à ¢Ã‹â€ Ã¢â‚¬  CTq ]/[ K(1+E) -à ¢Ã‹â€ Ã¢â‚¬  CTcb ]} =(1+E) -à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  CT Here -à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  CT = -(à ¢Ã‹â€ Ã¢â‚¬  CTq -à ¢Ã‹â€ Ã¢â‚¬  CT.cb) For amplicons which are designed less than 150 bp, primer and magnesium concentration are optimised, the efficiency is nearly equal to 1. Therefore the amount of target gene, normalized to an endogenous reference which is relative to a calibrator, taken as Amount of target =2-à ¢Ã‹â€ Ã¢â‚¬  Ãƒ ¢Ã‹â€ Ã¢â‚¬  CT . When the threshold is set for n standard deviations in the plot , above the mean of the base line emission from cycles 0 to n. The standard deviations is calculated from the data attained from base line of the amplification plot. When the fluorescence exceeds the threshold limit, CT values are determined at that point. The CT values are ten times more than the standard deviation of base line. The point at which amplification plot crosses the threshold can be defined as CT (Heid et al, 1996). Materials and methods Identifying pseudomonas aeruginosa Gram straining: Prepare a small amount of smear on a clear glass slide. Heat fix the smear to slide. Add a drop of crystal violet on to smear allow for 60 seconds. Rinse the slide with water. Add a drop of grams iodine to the smear allow for 60 seconds. Rinse the slide with water. Add 95% absolute ethanol to destain the crystal violet. Rinse again with water. Add a drop of saffronin over the smear, allow for 60 seconds. Rinse with water. Blot dry the slide, do not rub. Observe the smear under microscope. 10. If the colour of the bacteria appears pink it is gram negative, it is gram positive if it appears purple. Rod shaped pink coloured bacteria is clearly observed for gram negative pseudomonas aeruginosa. Shake flask method Prepare media of nutrient broth about 13 grams per litre of distilled water. Mix well and pour 50 ml of media into a 250ml conical flask. Plug the conical flask and wrap the mouth with aluminium foil. The less volumes of media in the flask increases surface area and oxygen transfer rate(OTR). The flasks are autoclaved at 1210 C and 1.5 lbs pressure for 1 hour 15 minutes. Planktonic-contains 50 ml of nutrient broth Biofilm-contains 25ml of PBS with microcarriers plus 25 ml of nutrient broth. Inoculate a loopful of pseudomonas aeruginosa into 50 ml nutrient broth aseptically. Allow the bacteria to grow overnight. Take the optical density at 550nm using spectrophotometer.The amount of the inoculums to be added to the shake flasks for analysis is calculated using the formula below: Amount of sample to add into shake flask = [working concentration X volume(ml)] / stock(OD) obtained After inoculation the flasks are kept in the orbital shaker at 300C. The samples of 1 ml is collected for RNA extraction at particular time intervals at 0,1,2, 4,24 and 48 hours for planktonic. In respect the biofilm samples are collected at time 0,22,24,26,44,46 and 48 hours as their growth is slower than the freely suspended planktonic cells. Then 2 ml of RNA later solution is added to the collected samples for RNA stabilization. The optical density is regularly monitored to check the growth of pseudomonas aeruginosa . Fermentation The fermentation process we perform here is batch fermentation. The bioreactor used for the study is a small scale fermentor from APPLICON BIOTECHNOLOGIES. The process will take place in a 3L fermentor(consists the working volume of 2.5L) for about 24 hours. During the run the criteria of temperature is kept at 300C . The pH is run free, stirrer speed is set at 600rpm for planktonic culture. The fermentor is run free for 24 hours overnight to check the production of foam. The cold finger helps to control the temperature by feeding water from/to the reactor. The o ring is checked for any leakage. The sample port is a tube for sampling using back pressure and forces the liquid into a glass recipient. The inlets for acid, alkali and antifoam are linked to durham bottles, going through the pumps. The durham bottles are blank, because above parameters are not controlled in this process. Air is pumped through a filter. The condenser is connected to water that comes out of the fermentor. Wh en the water is warmer than outside, condensed water reaches the condenser. The gas goes from an outlet connected to the condenser. The dissolved oxygen probe is connected to dO2 cable from the machine. It measures the amount of dO2 in the media. This probe needs to be kept in the buffer, when not in use. The probe should be plugged for 10 to 12 hours before the inoculation inorder to polarize. The stirrer is placed on the top of fermentor. The vessel is heated with the help of heating pad and temperature is detected using temperature probe. The air inlet is set up through the pumps and its volume is controlled with a rotometer in front of the machine. In this case 2.5L of air/min is supplied to the fermentor. For the gas analysis of oxygen and carbon dioxide the gas is passed through a drying column and reaches the monitor which is already set up for calibration. we run two runs of fermentor for both planktonic and biofilm cultures. The parameters such as temperature, aeration for planktonic and biofilm cultures are almost same. The only parameter varies for both is stirring is maintained at 600rpm for planktoni c and 200rpm for biofilm fermentation. Procedure: The fermentor is sterilised and all the valves are sealed with foil before going into the autoclave. The fermentor is run for about 24 hours before adding the inoculum to check any formation of foam. About 20 ml of an overnight culture is added to the fermentor. Sampling :About 7ml of sample is collected for every 20min for planktonic and every 2 hours for biofilm until the exponential phase is reached. The sample collected is preserved in the freezer for different analysis. The sample is assayed for gene expression by adding 2 ml of RNA later solution for RNA stability. The samples are assayed for ammonia, phosphorus, glucose and protease. The fermentation conditions are listed below. RNA EXTRACTION (protocol provided by applied biosystems) Materials required: 2-mercapto ethanol,100% ethanol, 10% SDS(in RNase free water), 0.5  µl/sample. Lysozyme solution: 100  µl/ sample 10mM Tris HCl (PH 8.0)mM EDTA 10mg/ml lysozyme (in RNase free water) Lysis buffer: Before beginning the lysis and homogenization steps, prepare a fresh amount of lysis buffer containing 1% 2-mercapto ethanol for each purification procedure. Add 10 µl 2-mercaptoethanol for each 1ml lysis buffer. Use 350  µl of freshly prepared lysis buffer for 1109 bacterial cells. Lysis and homogenization: Harvest 1109 bacterial cells and transfer them to an appropriately sized microcentrifuge tubes. Centrifuge at 500xg for 5 minutes at 40C to pellet cells. Discard the supernatant. Add 100  µl of prepared lysozyme solution to the cell pellet and resuspend by vortexing. Add 0.5  µl 10% SDS solution vortex to mix well. Incubate the cells in the tube for 5 minutes at room temperature. Add 350  µl lysis buffer prepared with 2-mercaptoethanol. Vortex to mix well. Transfer the lysate to a homogenizer inserted in an RNase free tube and centrifuge at 12,000xg for 2 minutes at room temperature. Remove the homogenizer when done. Purifying the RNA from bacterial cells: These steps are followed to bind wash and elute the RNA from the P.aeruginosa cells sample. Add 250  µl 100% ethanol to each volume of bacterial cell homogenate. Mix thoroughly by vortexing to dispersing any visible precipitate that may form after adding ethanol. Transfer the sample(including any remaining precipitate) to a spin cartridge( with a collection tube. Centrifuge both the spin cartridge and collection tube at 12,000xg for 15 seconds at room temperature. Discard the flow through and re-insert the spin cartridge in the same collection tube. Add 700  µl of wash buffer I to the spin cartridge. Centrifuge at 12,000xg for 15 seconds at room temperature. Discard the flow through and the collection tube. Place the spin cartridge into a new collection tube. Add 500  µl wash buffer II with ethanol to the spin cartridge. Centrifuge at 12,000Xg for 15 seconds at room temperature. Discard the flow through and re insert the spin cartridge into the same collection tube. The steps are repeated once again. Centrifuge the spin cartridge and collection tube at 12,000Xg for 1 minute at room temperature to dry the membrane with attached RNA. Discard the collection tube and insert the spin cartridge into a recovery tube. Add 50  µl of RNase free water to the centre of spin cartridge. Incubate at room temperature for 1 minute. Centrifuge the spin cartridge and recovery tube for 2 minutes at 12,000Xg at room temperature. Store the purified RNA or proceeded for DNase treatment after RNA treatment. (store purified RNA at -800C for long term). Agarose gel electrophoresis: The RNA samples after extraction are checked for the RNA signal. To visualise the RNA 1% agarose gel electrophoresis. The materials required for agarose gel electrophoresis is TAE buffer and agarose. TAE buffer(50x Tris acetate) 242 g of Tris base 57.1 ml glaciel acetic acid 100 ml of EDTA 0.5M PH .8.0 700ml of dH2O . About 20 ml of above solution is mixed with 980ml of distilled water to make it 1X. 1 gram of agarose is added to 100 ml of TAE buffer. The solution is mixed well. The solution is heated until the agarose is completely dissolved and solution becomes colourless. About 2.5  µl of ethidium bromide is added to the solution to visualise the RNA bands. Special care is taken while adding the ethidium bromide because it is bio-hazardous. Then about 5  µl RNA sample is mixed with 1  µl of DNA loading dye. The RNA samples are RUN with the potential difference of 80V with DNA Hyper ladder II as a standard. DNase treatment (protocol and kit provided by applied biosystem) The DNase I treatment is performed to remove any traces of genomic DNA from the eluted RNA. Protocol: Add 10x DNase buffer and DNase I to the RNA sample in the following composition: Amount component 1/9 th volume 10x DNase buffer 4  µl DNase I The components are mixed with gentle pippeting. Then the mixture is incubated at 370C for 90 minutes. DNase inactivation reagent is added to the solution and mixed well, leave at room temperature for 2 minutes. The samples are centrifuged at maximum speed for 1 minute to pellet the DNase inactivation reagent, after that the RNA solution is transferred to a new RNase free tube. The samples are run on 1% agarose gel electrophoresis to check the DNA contamination. We fou